Show simple item record

AuthorFischer, Susandc.contributor.author
Date of accession2016-03-15T06:23:22Zdc.date.accessioned
Available in OPARU since2016-03-15T06:23:22Zdc.date.available
Year of creation2009dc.date.created
AbstractIn this thesis three proteins in connection to the cellular RNA metabolism of the model archaeon H. volcanii were investigated for their biological functions. The first project focussed on gaining knowledge about the Lsm protein through generation and characterisation of a deletion mutant of the hvolsm gene as well as co-immunoprecipitation of potential interaction partners of the protein. While in Bacteria and Eukarya functions of the Sm/Lsm family of proteins are well understood, for the archaeal homologs only the typical ring-shaped structure and the characteristic binding to oligo(U) stretches could be shown thus far. This thesis research provides evidence that HvoLsm is not essential for cell survival. In addition, first co-immunoprecipitation experiments showed an interaction of HvoLsm with as yet unidentified RNA species as well as MS-analysed specific proteins. In another project the tRNA 3’ processing enzyme tRNase Z was investigated for its in vivo functions. Here, essentiality of this protein for survival of H. volcanii was verified, as a deletion of the hvotrz gene turned out to be lethal. Therefore the analysis of the biological function of this protein can only be achieved using different strategies (see Hölzle et al., 2008). In the last project dealing with the tRNase Z homolog NZ, it could be demonstrated that despite extensive in vitro and in vivo studies the function of this so far uncharacterised, unessential protein remains elusive. Nevertheless, several hints were obtained for an involvement of HvoNZ in the methylglyoxal metabolism or more specific the glyoxalase system as a glyoxalase II. Moreover, first results of co-immunoprecipitation experiments hint on a possible role in transcription regulation or even cell proliferation. Further analyses are necessary to account for the function and relevance of HvoNZ for the cell and possibly also for the evolution of the long version of the tRNase Z proteins.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandard (Fassung vom 01.10.2008)dc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v2dc.rights.uri
KeywordArchaeadc.subject
KeywordLsm-Proteindc.subject
KeywordNZ-Proteindc.subject
KeywordtRNase Zdc.subject
Dewey Decimal GroupDDC 580 / Botanical sciencesdc.subject.ddc
MeSHRNAdc.subject.mesh
TitleCharakterisierung von Proteinen des RNA-Metabolismus in Haloferax volcaniidc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-1831dc.identifier.doi
PPN621072052dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-72165dc.identifier.urn
GNDArchaebakteriendc.subject.gnd
GNDHaloferax volcaniidc.subject.gnd
FacultyFakultät für Naturwissenschaftenuulm.affiliationGeneral
Date of activation2010-02-22T14:55:10Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionZ: J-H 13.550; W: W-H 11.975uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS ID7216uulm.vtsID
CategoryPublikationenuulm.category
Bibliographyuulmuulm.bibliographie


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record