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AuthorEhrlich, Stevedc.contributor.author
Date of accession2016-03-14T15:23:06Zdc.date.accessioned
Available in OPARU since2016-03-14T15:23:06Zdc.date.available
Year of creation2009dc.date.created
AbstractAmplifications of the c-Rel locus on chromosome 2p13-p16 have been described in distinct lymphoma subtypes, most notably in mediastinal diffuse large B cell lymphoma (DLBCL) and classical Hodgkin´s lymphoma. In B cell lymphomas of the gastrointestinal tract gains of chromosomal material in chromosomal region 2p13-p16 could also be detected using comparative genomic hybridization (CGH). Candidate genes for lymphomagenesis in this region are c-Rel and BCL11A. The objective of this work was to shape out a potential gene dosis effect. For this purpose, 13 lymphomas of the gastrointestinal tract (8 diffuse large cell B cell lymphoma and 5 marginal zone B cell lymphoma) have been analyzed for their genomic status of c-Rel and BCL11A using fluorescence in situ hybridization (FISH). For 3 out of 13 lymphomas a gain of chromosomal material in chromosomal region 2p13-p16 had been detected. By using FISH a gain of gene number copies could be detected 5 lymphomas (3 of which had known gains of chromosomal material in CGH). The relative expression of c-Rel and BCL mRNA was detected using quantitative real time PCR. Furthermore, the expression and intracellular localization of the c-Rel and BCL11A protein were confirmed by using immunohistochemical staining with antibodies not commercially available. There was evidence for a stringen t gene dosis effect of c-Rel and BCL11A in only 2 out of 13 lymphomas. The remaining lymphomas present a heterogenous expression pattern. Maybe there are other regulatory mechanisms leading to constitutive activation of c-Rel and/ or BCL11A.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandard (Fassung vom 01.10.2008)dc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v2dc.rights.uri
KeywordBCL11Adc.subject
Keywordc-Reldc.subject
KeywordGastrointestinal B cell lymphomadc.subject
KeywordGene dosis effectdc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHCarrier proteinsdc.subject.mesh
MeSHGenes, reldc.subject.mesh
MeSHLymphoma, B-celldc.subject.mesh
TitleAnalyse des genomischen Status der Proto-Onkogene c-Rel und BCL11A sowie deren RNA- und Proteinexpression in Marginalzonen-B-Zell-Lymphomen und diffus großzelligen B-Zell-Lymphomen des Gastrointestinaltraktesdc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-1593dc.identifier.doi
PPN59974930Xdc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-67942dc.identifier.urn
GNDB-Zell-Lymphomdc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
Date of activation2009-05-08T16:43:48Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionZ: J-H 13.264; W: W-H 11.703uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS ID6794uulm.vtsID
CategoryPublikationenuulm.category
Bibliographyuulmuulm.bibliographie


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