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AuthorSchmid, Oliverdc.contributor.author
Date of accession2016-03-14T15:22:56Zdc.date.accessioned
Available in OPARU since2016-03-14T15:22:56Zdc.date.available
Year of creation2009dc.date.created
AbstractFormaldehyde (FA) is an important industrial compound. It has also applications as a disinfectant and preservative. Furthermore, FA is naturally occurring in cells, tissues and body fluids. It is known to be genotoxic; the primary DNA alterations induced by FA are DNA-protein crosslinks (DPX). The aim of this work was to characterize various aspects of the genotoxicity of FA in vitro and in vivo. The concentration-effect relationship of genotoxic effects of FA in three genotoxicity tests was characterized by fitting different curves to the data. The results suggest a thresholded mechanism of FA-induced genotoxic effects. The induction and the repair of FA-induced DPX were investigated in A549 human lung cells and primary human nasal epithelial cells. The susceptibility of both cell types for the induction of DPX is very similar. DPX are repaired by these cells within about 8 hours. The expression of FA-metabolizing enzymes (Glutathion-dependant FA dehydrogenase, aldehyd-dehydrogenase 1A1, aldehyd-dehydrogenase 2) in A549 cells was analyzed after FA-exposure for 1,4 and 24 hours by real time PCR. No significant alterations were measured. The induction of genotoxic effects by FA was investigated in human blood cultures. The results show that high amounts of DPX are needed at the start of the culture to induce cytogenetic effects in lymphocytes. Although several biomonitoring studies reported genotoxic effects in the blood of subjects exposed to FA, the results shown here suggest that it is very unlikely that such effects are actually related to FA-exposure. An inhalation study with human volunteers was conducted to assess local mutagenic effects of FA by the micronucleus test with exfoliated buccal mucosa cells. FA peak exposure was up to 1 ppm and the cumulative dose was 13,5 ppm h over two weeks. The results indicate that under these exposure conditions, FA does not induce micronuclei in buccal mucosa cells.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandard (Fassung vom 01.10.2008)dc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v2dc.rights.uri
KeywordDNA-protein crosslinksdc.subject
KeywordDPXdc.subject
KeywordGenotoxicitydc.subject
KeywordSisterchromatid exchangedc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHCytotoxicity, immunologicdc.subject.mesh
MeSHFormaldehydedc.subject.mesh
MeSHMicronucleus testsdc.subject.mesh
TitleUntersuchungen zur Genotoxizität von Formaldehyd in vitro und in vivodc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-1560dc.identifier.doi
PPN1647661552dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-66943dc.identifier.urn
GNDComet Assaydc.subject.gnd
GNDFormaldehyddc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
Date of activation2009-01-30T11:28:47Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionZ: J-H 13.215; W: W-H 11.659uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS-ID6694uulm.vtsID
CategoryPublikationenuulm.category
University Bibliographyjauulm.unibibliographie


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