Wirkung von Hypericin-induzierter PDT auf die intrazelluläre Ca2+-Konzentration humaner Glioblastomzellen
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During photodynamic therapy (PDT) the interaction between light and photosensitizers induces oxidative stress and the production of reactive oxygen species (ROS). ROS formation influences ion channels and causes changes in the Ca²+ concentration which may induce further reactions leading to cell stimulation or cell death. In this study we analysed the influence of irradiation conditions on the cell death mechanisms during Hypericin-induced PDT. The process of apoptosis is characterised by morphological and physiological changes of the cell membrane and of the whole intracellular chain reactions. The translocation of the phospholipid phosphatidylserine from the inner to the outer leaflet of the plasma membrane is one of the earliest indications of apoptotic cell death. The translocation was traced by the binding of FITC (fluorescein isothiocyanate) conjugated Annexin-V to phosphatidilserine and FACS (fluorescence activated cell sorting) analysis.
Subject HeadingsApoptosis [GND]
Photodynamische Therapie [GND]