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AuthorVöhringer, Sabinedc.contributor.author
Date of accession2016-03-14T15:22:46Zdc.date.accessioned
Available in OPARU since2016-03-14T15:22:46Zdc.date.available
Year of creation2006dc.date.created
AbstractGenetic immunization is a new approach in the development of vaccination strategies against infectious diseases. It involves the delivery of nucleic acids into target host cells for the expression of pathogen-derived antigens in situ to elicit a protective humoral and cell-mediated immune response. Several viral and non-viral gene transfer systems for the delivery of nucleic acids can be employed. Among these adenovirus vectors (Ad vectors) show great promise since they can infect a wide variety of dividing and non-dividing cells, do not integrate into the host cell genome and offer a high capacity for the genetic incorporation of transgenes. However, problems may arise due to pre-existing immunity and non-specific transduction of host cells. To address these problems a novel platform for so-called "genetichemical" capsid modification of Ad vectors has been developed that allows for modifying vector tropism and shielding. Objective of the work presented here was to construct non-modified and "genetichemically" modified Ad vectors which express the model antigens HBsAg and ovalbumin that can be used to study immune responses after genetic vaccination. The vectors were produced and characterized by genetic and biochemical techniques. Furthermore, vectors were injected into mice by different administration routes and immune responses to the expressed transgenes were evaluated.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandard (Fassung vom 01.10.2008)dc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v2dc.rights.uri
KeywordAdenovirus vectorsdc.subject
KeywordHBsAgdc.subject
KeywordPEGylationdc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHHepatitis B surface antigensdc.subject.mesh
MeSHVaccinationdc.subject.mesh
TitleKonstruktion und Charakterisierung Serotyp 5-basierter adenoviraler Vektoren zur Expression von Modellantigenen für genetische Vakzinierungdc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-1532dc.identifier.doi
PPN591154862dc.identifier.ppn
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-66358dc.identifier.urn
GNDHepatitis-B-Surface-Antigendc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
Date of activation2008-12-23T09:57:36Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionZ: J-H 13.136; W: W-H 11.582uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS ID6635uulm.vtsID
CategoryPublikationenuulm.category
Bibliographyuulmuulm.bibliographie


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