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AutorWölfle, Martinadc.contributor.author
Aufnahmedatum2016-03-14T15:22:13Zdc.date.accessioned
In OPARU verfügbar seit2016-03-14T15:22:13Zdc.date.available
Jahr der Erstellung2007dc.date.created
ZusammenfassungHere the phenotype and differentiation capacity of human adult hippocampal NPCs (hNPCs), derived from patients who underwent epilepsy surgery, is characterized. Isolated hNPCs were cultured in clonal density by transferring the cells to serum-free media supplemented with FGF-2 and EGF in 3 % atmospheric oxygen. The hNPCs showed neurosphere formation, expressed high levels of early neuroectodermal markers like NeuroD1 and Olig2, the NSC markers Nestin and Musashi1 and the proliferation marker Ki67. hNPCs spontaneously differentiated into a neuronal, astroglial and oligodendroglial phenotype. Differentiated hNPCs showed functional properties of neurons, such as sodium channels, action potentials and production of the neurotransmitters glutamate and GABA. Additionally a global gene expression profiling was performed using RNA from adult human hippocampus-derived neuroprogenitor cells (NPCs) and multipotent frontal cortical fetal NPCs in comparison to adult human mesenchymal stem cells (hMSCs) as a multipotent adult stem cell control, and adult human hippocampal tissue, to define a gene expression pattern which is specific for human NPCs. The results were compared with data from various databases. Hierarchical cluster analysis of all neuroectodermal cell/tissue types revealed a strong relationship of adult hippocampal NPCs with various white matter tissues, while fetal NPCs strongly correlate with fetal brain tissue. However, adult and fetal NPCs share the expression of a variety of genes known to be related to signal transduction, cell metabolism and neuroectodermal tissue. In contrast, adult NPCs and hMSCs overlap in the expression of genes mainly involved in extracellular matrix biology. This provides a framework for standardized comparative gene expression analysis of human brain-derived NPCs with other stem cell populations or differentiated tissues.dc.description.abstract
Spracheendc.language.iso
Verbreitende StelleUniversität Ulmdc.publisher
ID Originalpublikationhttp://stemcells.alphamedpress.org/cgi/content/full/25/5/1231dc.relation.uri
LizenzStandard (Fassung vom 03.05.2003)dc.rights
Link zum Lizenztexthttps://oparu.uni-ulm.de/xmlui/license_v1dc.rights.uri
SchlagwortAdult neural stem cellsdc.subject
DDC-SachgruppeDDC 610 / Medicine & healthdc.subject.ddc
MeSHAdult stem cellsdc.subject.mesh
MeSHMesenchymal stem cellsdc.subject.mesh
TitelComparative analyses of the neurogenic capacity of human neuroprogenitor populations derived from neural and mesodermal tissuedc.title
RessourcentypDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-1396dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-63715dc.identifier.urn
GNDMesodermdc.subject.gnd
GNDNervenstammzelledc.subject.gnd
GNDNeurogenesedc.subject.gnd
FakultätMedizinische Fakultätuulm.affiliationGeneral
Quelle OriginalpublikationStem cells, 25.2007, S. 1231 - 1240uulm.citationOrigPub
Datum der Freischaltung2008-04-30T09:52:44Zuulm.freischaltungVTS
Peer-Reviewjauulm.peerReview
Signatur DruckexemplarZ: J-H 11.793; W: W-H 9.990uulm.shelfmark
DCMI MedientypTextuulm.typeDCMI
VTS-ID6371uulm.vtsID
KategoriePublikationenuulm.category


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