Show simple item record

AuthorWölfle, Martinadc.contributor.author
Date of accession2016-03-14T15:22:13Zdc.date.accessioned
Available in OPARU since2016-03-14T15:22:13Zdc.date.available
Year of creation2007dc.date.created
AbstractHere the phenotype and differentiation capacity of human adult hippocampal NPCs (hNPCs), derived from patients who underwent epilepsy surgery, is characterized. Isolated hNPCs were cultured in clonal density by transferring the cells to serum-free media supplemented with FGF-2 and EGF in 3 % atmospheric oxygen. The hNPCs showed neurosphere formation, expressed high levels of early neuroectodermal markers like NeuroD1 and Olig2, the NSC markers Nestin and Musashi1 and the proliferation marker Ki67. hNPCs spontaneously differentiated into a neuronal, astroglial and oligodendroglial phenotype. Differentiated hNPCs showed functional properties of neurons, such as sodium channels, action potentials and production of the neurotransmitters glutamate and GABA. Additionally a global gene expression profiling was performed using RNA from adult human hippocampus-derived neuroprogenitor cells (NPCs) and multipotent frontal cortical fetal NPCs in comparison to adult human mesenchymal stem cells (hMSCs) as a multipotent adult stem cell control, and adult human hippocampal tissue, to define a gene expression pattern which is specific for human NPCs. The results were compared with data from various databases. Hierarchical cluster analysis of all neuroectodermal cell/tissue types revealed a strong relationship of adult hippocampal NPCs with various white matter tissues, while fetal NPCs strongly correlate with fetal brain tissue. However, adult and fetal NPCs share the expression of a variety of genes known to be related to signal transduction, cell metabolism and neuroectodermal tissue. In contrast, adult NPCs and hMSCs overlap in the expression of genes mainly involved in extracellular matrix biology. This provides a framework for standardized comparative gene expression analysis of human brain-derived NPCs with other stem cell populations or differentiated tissues.dc.description.abstract
Languageendc.language.iso
PublisherUniversität Ulmdc.publisher
ID of original publ.http://stemcells.alphamedpress.org/cgi/content/full/25/5/1231dc.relation.uri
LicenseStandard (Fassung vom 03.05.2003)dc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v1dc.rights.uri
KeywordAdult neural stem cellsdc.subject
Dewey Decimal GroupDDC 610 / Medicine & healthdc.subject.ddc
MeSHAdult stem cellsdc.subject.mesh
MeSHMesenchymal stem cellsdc.subject.mesh
TitleComparative analyses of the neurogenic capacity of human neuroprogenitor populations derived from neural and mesodermal tissuedc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-1396dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-63715dc.identifier.urn
GNDMesodermdc.subject.gnd
GNDNervenstammzelledc.subject.gnd
GNDNeurogenesedc.subject.gnd
FacultyMedizinische Fakultätuulm.affiliationGeneral
Citation of original publ.Stem cells, 25.2007, S. 1231 - 1240uulm.citationOrigPub
Date of activation2008-04-30T09:52:44Zuulm.freischaltungVTS
Peer reviewjauulm.peerReview
Shelfmark print versionZ: J-H 11.793; W: W-H 9.990uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS-ID6371uulm.vtsID
CategoryPublikationenuulm.category


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record