Comparative analyses of the neurogenic capacity of human neuroprogenitor populations derived from neural and mesodermal tissue
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Here the phenotype and differentiation capacity of human adult hippocampal NPCs (hNPCs), derived from patients who underwent epilepsy surgery, is characterized. Isolated hNPCs were cultured in clonal density by transferring the cells to serum-free media supplemented with FGF-2 and EGF in 3 % atmospheric oxygen. The hNPCs showed neurosphere formation, expressed high levels of early neuroectodermal markers like NeuroD1 and Olig2, the NSC markers Nestin and Musashi1 and the proliferation marker Ki67. hNPCs spontaneously differentiated into a neuronal, astroglial and oligodendroglial phenotype. Differentiated hNPCs showed functional properties of neurons, such as sodium channels, action potentials and production of the neurotransmitters glutamate and GABA. Additionally a global gene expression profiling was performed using RNA from adult human hippocampus-derived neuroprogenitor cells (NPCs) and multipotent frontal cortical fetal NPCs in comparison to adult human mesenchymal stem cells (hMSCs) as a multipotent adult stem cell control, and adult human hippocampal tissue, to define a gene expression pattern which is specific for human NPCs. The results were compared with data from various databases. Hierarchical cluster analysis of all neuroectodermal cell/tissue types revealed a strong relationship of adult hippocampal NPCs with various white matter tissues, while fetal NPCs strongly correlate with fetal brain tissue. However, adult and fetal NPCs share the expression of a variety of genes known to be related to signal transduction, cell metabolism and neuroectodermal tissue. In contrast, adult NPCs and hMSCs overlap in the expression of genes mainly involved in extracellular matrix biology. This provides a framework for standardized comparative gene expression analysis of human brain-derived NPCs with other stem cell populations or differentiated tissues.
Original publicationStem cells, 25.2007, S. 1231 - 1240
Subject HeadingsMesoderm [GND]
Adult stem cells [MeSH]
Mesenchymal stem cells [MeSH]