• English
    • Deutsch
  • English 
    • English
    • Deutsch
  • Login
View Item 
  •   Home
  • Universität Ulm
  • Publikationen
  • View Item
  •   Home
  • Universität Ulm
  • Publikationen
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Functional analysis of ecdysone receptor

Thumbnail
vts_6868_9525.pdf (1.569Mb)
100 Seiten
Veröffentlichung
2009-06-21
Authors
Azoitei, Anca
Dissertation


Faculties
Fakultät für Naturwissenschaften
Abstract
Ecdysteroids are important regulators of insect development. They perform their actions through intracellular receptors belonging to the superfamily of nuclear receptors (NRs). In the present study, the relative performance of the three Drosophila ecdysone receptor (EcR)-isoforms in terms of their affinity to the ligand Ponasterone A in mammalian cell line CHO-K1 was determined. In the absence of a heterodimerization partner hormone binding of EcR is rather weak. The presence of ecdysone response elements (EcREs) stimulates the ligand binding to different degrees depending on the EcR-isoform involved. When Ultraspiracle (Usp), the invertebrate orthologue of mammalian RXR, is used as dimerization partner, all EcR isoforms of Drosophila bind the ligand Ponasterone A with the same high affinity already in the absence of EcREs. Depending on the EcR isoform, Usp variant and EcREs, addition of DNA results in augmented ligand binding to the heterodimer EcR/Usp. In the absence of EcREs, hormone binding is not affected if the A/B domain of wild type Usp is replaced by the activation domain (AD) of herpex simplex virus (VP16) to circumvent the inhibition of transcriptional activity of the wild type Usp. By contrast, substantial differences were observed with VP16-Usp fusion proteins in the presence of EcREs. In contrast to Usp, the mammalian ortholog-RXR confers ligand affinity to the receptor complex only in the presence of an EcRE. Finally, specific mutations in the EcR ligand binding domain (LBD) showed an abolishing effect on the ligand binding function of this receptor.
Date created
2009
Subject headings
[GND]: Ecdysone | Ecdysteroide
[LCSH]: DNA-binding proteins
[Free subject headings]: Hormone binding | Ponasterone
[DDC subject group]: DDC 570 / Life sciences
License
Standard (Fassung vom 01.10.2008)
https://oparu.uni-ulm.de/xmlui/license_v2

Metadata
Show full item record

DOI & citation

Please use this identifier to cite or link to this item: http://dx.doi.org/10.18725/OPARU-1251

Azoitei, Anca (2009): Functional analysis of ecdysone receptor. Open Access Repositorium der Universität Ulm und Technischen Hochschule Ulm. Dissertation. http://dx.doi.org/10.18725/OPARU-1251
Citation formatter >



Policy | kiz service OPARU | Contact Us
Impressum | Privacy statement
 

 

Advanced Search

Browse

All of OPARUCommunities & CollectionsPersonsInstitutionsPublication typesUlm SerialsDewey Decimal ClassesEU projects UlmDFG projects UlmOther projects Ulm

My Account

LoginRegister

Statistics

View Usage Statistics

Policy | kiz service OPARU | Contact Us
Impressum | Privacy statement