Mid-infrared thin-film diamond waveguides combined with tunable quantum cascade lasers for analyzing the secondary structure of proteins

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Erstveröffentlichung
2016-04-28
Wissenschaftlicher Artikel
Authors
Lopez-Lorente, Angela I.
Wang, Pei
Sieger, Markus
Catalan Vargas, Ernesto
Karlsson, Mikael
Faculties
Fakultät für NaturwissenschaftenInstitutions
Institut für Analytische und Bioanalytische ChemiePublished in
physica status solidi (a) ; 213 (2016), 8. - S. 2117-2123. - ISSN 0031-8965. - eISSN 1862-6319
Link to original publication
https://dx.doi.org/10.1002/pssa.201600134Peer review
ja
Document version
acceptedVersion
Abstract
Diamond has excellent optical properties including broadband transmissivity, low self-absorption, and a high refractive index, which have prompted its use for optical sensing applications. Thin- film diamond strip waveguides (DSWGs) combined with tunable quantum cascade lasers (tQCLs) providing an emission wave- length range of 5.78–6.35 mm (1735–1570 cm"1) have been used to obtain mid-infrared (MIR) spectra of proteins, thereby enabling the analysis of their secondary structure via the amide I band. Three different proteins were analyzed, namely bovine serum albumin (BSA), myoglobin, and g-globulin. The secondary structure of BSA and myoglobin has a major contribution of a-helices, whereas g-globulins are rich in b-sheet structures, which is reflected in the amide I band. A comparison of the spectra obtained via the combination of the tQCL and DSWG with spectra obtained using conventional Fourier transform infrared (FTIR) spectroscopy and a commercial diamond attenuated total reflection (ATR) element has been performed. It is shown that the main features evident in FTIR-ATR spectra are also obtained using tQCL-DSWG sensors.
EU Project
TROPSENSE / Development of a non-invassive breath test for early diagnosis of tropical diseases / EC / H2020 / 645758
Earlier version(s)
http://dx.doi.org/10.18725/OPARU-6079Subject Headings
Infrarotdetektor [GND]Infrarotspektroskopie [GND]
Proteine [GND]
Quantenkaskadenlaser [GND]
Wellenleiter [GND]
Diamant [GND]
Sekundärstruktur [GND]
ATR-Technik [GND]
Infrared detectors [LCSH]
Infrared spectroscopy [LCSH]
Proteins [LCSH]
Lasers [LCSH]
Wave guides [LCSH]
Diamonds [LCSH]
Keywords
Diamond; Waveguides; Infrared sensors; Quantum cascade lasers; Infrared Spectroscopy; ProteinsDewey Decimal Group
DDC 540 / Chemistry & allied sciencesMetadata
Show full item recordCitation example
Lopez-Lorente, Angela I. et al. (2019): Mid-infrared thin-film diamond waveguides combined with tunable quantum cascade lasers for analyzing the secondary structure of proteins. Open Access Repositorium der Universität Ulm. http://dx.doi.org/10.18725/OPARU-12426