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AuthorWanischeck, Mariodc.contributor.author
Date of accession2016-03-14T15:20:32Zdc.date.accessioned
Available in OPARU since2016-03-14T15:20:32Zdc.date.available
Year of creation2008dc.date.created
AbstractIt is essential for excitable cells to keep their activity within a physiological and appropriate range independently from changes in synaptic efficacy or cellular excitability which can occur during phases of learning and/or development. Recently shown results indicated that excitable cells like neurons and muscles are capable of regulating their own activity. Most of those studies, however showed compensatory effects at the level of synaptic efficacy. Those showing changes in ion channel expression to maintain cellular excitability are rare and therefore less understood. Using the advantages of the Drosophila Gal4/UAS-System I generated muscle-specific mutant larvae that expressed a modified Shaker-potassium channel leading to a dramatically decreased cellular excitability. The aim of this work was to detect possible compensatory changes at the level of ion channels and study the underlying cellular mechanisms. Measurements of muscle performance did not display severe impairments in the contractile properties (tension and kinetics) when mutants were compared with wildtypes thus suggesting some kind of compensatory changes. Additional experiments excluded changes of motor synaptic transmission and/or motoneuronal output. On the level of ion channels, an increased calcium current amplitude of about 30% was detected in mutant muscle cells, which was accompanied by an up-regulation of the corresponding calcium channel beta-subunit expression rate. Finally a different mutant was tested, which expressed the Shaker gene late in development and thus had only a limited time for possible compensation. In contrast to the "early" shaker-mutant, this "late" mutant showed impaired muscle performance. This experiment suggested that the expression of the genetically modified potassium channel leads to severe impairments of contractile properties if the effect had no time to be compensated by an increased calcium current.dc.description.abstract
Languagededc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandard (Fassung vom 03.05.2003)dc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v1dc.rights.uri
Dewey Decimal GroupDDC 570 / Life sciencesdc.subject.ddc
MeSHHomeostasisdc.subject.mesh
MeSHMusclesdc.subject.mesh
MeSHPlasticitydc.subject.mesh
TitleZellautonome, homöostatische Regulation der elektrischen Erregbarkeit von Körperwandmuskelzellen der Taufliege Drosophila melanogasterdc.title
Resource typeDissertationdc.type
DOIhttp://dx.doi.org/10.18725/OPARU-1135dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-vts-64009dc.identifier.urn
GNDTaufliegedc.subject.gnd
FacultyFakultät für Naturwissenschaftenuulm.affiliationGeneral
Date of activation2008-06-09T08:33:25Zuulm.freischaltungVTS
Peer reviewneinuulm.peerReview
Shelfmark print versionZ: J-H 11.848; W: W-H 11.338uulm.shelfmark
DCMI TypeTextuulm.typeDCMI
VTS ID6400uulm.vtsID
CategoryPublikationenuulm.category
Bibliographyuulmuulm.bibliographie


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