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AuthorLópez-Lorente, Angela I.dc.contributor.author
AuthorIzquierdo, Javierdc.contributor.author
AuthorKranz, Christinedc.contributor.author
AuthorMizaikoff, Borisdc.contributor.author
Date of accession2018-10-26T12:24:14Zdc.date.accessioned
Available in OPARU since2018-10-26T12:24:14Zdc.date.available
Date of first publication2017-07dc.date.issued
AbstractBoron-doped diamond (BDD) has been modified with bare gold nanoparticles (AuNPs) for the investigation of bovine serum albumin (BSA) protein films. First, the BDD electrode was amino- terminated via silanization with (3-aminopropyl)triethoxysilane (APTES). Then, the AuNPs were synthesized using a stainless steel-assisted method, and immobilized at the APTES-modified BDD surface (BDD/APTES/AuNPs). The resulting morphology of both modification steps was characterized using atomic force microscopy (AFM). Besides, the synthesis of AuNPs was in-situ monitored via infrared attenuated total reflection (IR-ATR) spectroscopy using a combined IR-ATR-EC-AFM system taking advantage of surface enhanced IR absorption (SEIRA) effects induced by the AuNPs. The modified conductive IR-ATR BDD waveguide was employed to investigate spectroelectrochemically films of globular and fibrilized BSA, which were deposited onto the AuNPs-modified surface. SEIRA spectroscopy enabled the discrimination between globular and fibril aggregates of BSA via curve fitting of amide I band revealing a major contributions of crossed b-sheet structures within the fibril aggregates. Finally, preliminary square-wave voltammetry experiments showed that the spectroelectrochemical setup has potential for in-situ electrochemical characterization of such protein films.dc.description.abstract
Languageen_USdc.language.iso
PublisherUniversität Ulmdc.publisher
LicenseStandarddc.rights
Link to license texthttps://oparu.uni-ulm.de/xmlui/license_v3dc.rights.uri
KeywordMid-infrared spectroscopydc.subject
KeywordIR-ATRdc.subject
KeywordGold nanoparticlesdc.subject
KeywordBoron-doped diamonddc.subject
Dewey Decimal GroupDDC 540 / Chemistry & allied sciencesdc.subject.ddc
LCSHElectrochemistrydc.subject.lcsh
LCSHAtomic force microscopydc.subject.lcsh
LCSHNanoparticlesdc.subject.lcsh
MeSHSerum albumin, bovinedc.subject.mesh
TitleBoron-doped diamond modified with gold nanoparticles for the characterization of bovine serum albumin proteindc.title
Resource typeWissenschaftlicher Artikeldc.type
VersionacceptedVersiondc.description.version
DOIhttp://dx.doi.org/10.18725/OPARU-10209dc.identifier.doi
URNhttp://nbn-resolving.de/urn:nbn:de:bsz:289-oparu-10266-4dc.identifier.urn
GNDMIR-Spektroskopiedc.subject.gnd
GNDElektrochemiedc.subject.gnd
GNDRasterkraftmikroskopiedc.subject.gnd
GNDNanopartikeldc.subject.gnd
GNDBordc.subject.gnd
FacultyFakultät für Naturwissenschaftenuulm.affiliationGeneral
InstitutionInstitut für Analytische und Bioanalytische Chemieuulm.affiliationSpecific
Peer reviewjauulm.peerReview
DCMI TypeTextuulm.typeDCMI
CategoryPublikationenuulm.category
Issueuulm.issue
DOI of original publication10.1016/j.vibspec.2016.10.010dc.relation1.doi
Source - Title of sourceVibrational spectroscopysource.title
Source - Place of publicationElseviersource.publisher
Source - Volume91source.volume
Source - Year2017source.year
Source - From page147source.fromPage
Source - To page156source.toPage
Source - ISSN0924-2031source.identifier.issn
EU projectTROPSENSE / Development of a non-invassive breath test for early diagnosis of tropical diseases / EC / H2020 / 645758uulm.projectEU
University Bibliographyjauulm.unibibliographie


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