Boron-doped diamond modified with gold nanoparticles for the characterization of bovine serum albumin protein
López-Lorente, Angela I.
FacultiesFakultät für Naturwissenschaften
InstitutionsInstitut für Analytische und Bioanalytische Chemie
Vibrational spectroscopy ; 91 (2017). - S. 147-156. - ISSN 0924-2031
Link to original publicationhttps://dx.doi.org/10.1016/j.vibspec.2016.10.010
Boron-doped diamond (BDD) has been modified with bare gold nanoparticles (AuNPs) for the investigation of bovine serum albumin (BSA) protein films. First, the BDD electrode was amino- terminated via silanization with (3-aminopropyl)triethoxysilane (APTES). Then, the AuNPs were synthesized using a stainless steel-assisted method, and immobilized at the APTES-modified BDD surface (BDD/APTES/AuNPs). The resulting morphology of both modification steps was characterized using atomic force microscopy (AFM). Besides, the synthesis of AuNPs was in-situ monitored via infrared attenuated total reflection (IR-ATR) spectroscopy using a combined IR-ATR-EC-AFM system taking advantage of surface enhanced IR absorption (SEIRA) effects induced by the AuNPs. The modified conductive IR-ATR BDD waveguide was employed to investigate spectroelectrochemically films of globular and fibrilized BSA, which were deposited onto the AuNPs-modified surface. SEIRA spectroscopy enabled the discrimination between globular and fibril aggregates of BSA via curve fitting of amide I band revealing a major contributions of crossed b-sheet structures within the fibril aggregates. Finally, preliminary square-wave voltammetry experiments showed that the spectroelectrochemical setup has potential for in-situ electrochemical characterization of such protein films.
TROPSENSE / Development of a non-invassive breath test for early diagnosis of tropical diseases / EC / H2020 / 645758
Subject HeadingsMIR-Spektroskopie [GND]
Atomic force microscopy [LCSH]
Serum albumin, bovine [MeSH]