Identification of B cell subpopulations with pro- and anti-tumorigenic properties in an immunocompetent mouse model of head and neck squamous cell carcinoma

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Date

2024-12-29

Authors

Sonntag, Michael
Stanojevic, Sandra
Laban, Simon
Schuler, Patrick J.
Hoffmann, Thomas K.
Brunner, Cornelia

Journal Title

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Publication Type

Wissenschaftlicher Artikel

Published in

Cells, 2024

Abstract

Abstract Due to their high developmental diversity and different regulatory and functional roles, B cell subpopulations can promote or inhibit tumor growth. An orthotopic murine HNSCC model was applied to investigate the B cell composition and function in HNSCCs. Using flow cytometry approaches, cells from the spleen, lymph nodes and tumors were analyzed. Additionally, immunoglobulin (Ig) levels post-tumor induction were tracked via enzyme-linked immunosorbent assays (ELISA). Following tumor induction, GCs, as well as increasing numbers of GL7 + CD95 + GC B cells in the spleen and tumor tissues, were detected. In parallel, we observed CD39 + CD73 + B cells in tumors and spleens of tumor-bearing mice. Notably, CD39 + CD73 + expression was primarily detected on MZ B cells and to a lesser extent on follicular (FO) and non-follicular, newly formed (NF) B cells, supposing an immunosuppressive function of MZ B cells in the TME. Parallel to increased MZ B cell numbers in secondary lymphoid organs (SLOs) as well as in the tumor tissue, IgM antibody (Ab) levels rose continuously. In contrast, IgG1, IgG2, and IgG3 levels increased at later time points. Understanding the complex interactions between B cell subsets and the TME could lead to new strategies for enhancing the treatment and prognosis of HNSCC patients.

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Faculties

Institutions

UKU. Klinik für Hals-, Nasen-, Ohrenheilkunde, Kopf- und Halschirurgie

Citation

DFG Project uulm

Der Einfluss von B-Zellen auf den therapeutischen Erfolg der Immuntherapie bei Patienten mit Kopf-Hals-Karzinom / DFG / 504016957 [BR 289111-1 SCHU 25639-1]

EU Project THU

Other projects THU

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CC BY 4.0 International

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DOI external

DOI external

10.3390/cells14010020

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DFG Project THU

item.page.thu.projectEU

item.page.thu.projectOther

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Keywords

B cells, HNSCC, Germinal centers, Adenosine, Immunocompetent mouse model, DDC 610 / Medicine & health